A clinician’s test selection and interpretation, diagnosis, and patient management decisions should be based on his/her education, clinical expertise, and assessment of the patient. Search clinical laboratory tests offered by the Department of Laboratory Medicine at the University of Washington Search within other orders version 11331bae Contact:. This FAQ is provided for informational purposes only and is not intended as medical advice. Performance of the Focus and Kalon enzyme-linked immunosorbent assays for antibodies to herpes simplex virus type 2 glycoprotein G in culture-documented cases of genital herpes. Detection of herpes simplex virus type 2-specific immunoglobulin G antibodies in African sera by using recombinant gG2, Western blotting, and gG2 inhibition. Herpes simplex virus type 2 (HSV-2) IgG index values in two immunoassays in relation to HSV-2 IgG inhibition assay results. Inhibition values >60% indicate true HSV-2-specific IgG reactivity Index value for serum pre-incubated with HSV-1 lysate = 2.45 Index value for serum pre-incubated with HSV-2 lysate = 0.23 An example of how the inhibition value is calculated is shown below: The HSV-1 lysate controls for any non-gG2 reactivity present in the serum sample thus, any inhibition that is detected reflects only the absorption of gG2 reactive antibodies by the native gG2 present in the HSV-2 lysate. The index value of serum pre-incubated with HSV-2 lysate is then compared to the index value of serum pre-incubated with HSV-1 lysate. 2 Separate aliquots of the patient sample are incubated with the 2 lysates, then tested in the HerpeSelect HSV-2 type-specific IgG ELISA. The HSV-2 IgG inhibition assay measures the differential abilities of lysates of cells infected with HSV-1 or HSV-2 to neutralize patient sample reactivity to recombinant gG2 protein (rgG2), the HSV-2–specific protein used in the HerpeSelect HSV-2 IgG ELISA.
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